Protocols • DNA Manipulation and Gel Electrophoresis

[Under Construction] This set of protocols covers basic techniques of DNA handling, separation, purification.

DNA Gel Electrophoresis

  • Agarose Gels
    • Sample preparation and gel running
    • Ethidium bromide staining
  • Native Polyacrylamide Gels
    • Sample preparation and gel running
    • SYBR green staining

DNA Manipulation

  • Ethanol precipitation
    • sodium acetate
    • ammonium acetate
  • Isopropanol precipitation
  • Phenol:chloroform extraction
  • Chloroform extraction
  • Gel-purification
    • Agarose
    • Polyacrylamide

scott@chem.uga.edu
Department of Chemistry
University of Georgia
Athens, GA 30602-2556
706 542-2240 | FAX: 706 542-2295